Zoisite has wavy auburn hair pulled back in a ponytail. The ponytail is held in a green band. Zoisite has a blue-grey suit with green trim. He has a thin black belt, tall black boots, white gloves, and small green stud earrings.
Zoisite's first appearance in the anime was during Nephrite's first active episode. He and Nephrite were invariably at odds, always taunting him when one of his plans failed, and becoming crestfallen when Nephrite was still in charge. He became more upset when Nephrite began directing his plans away from collecting energy and began searching for the Silver Crystal. A task Zoisite was charged with. Upon realizing that Nephrite betrayed the Dark Kingdom, Zoisite sent three of his personal Youma after him, one of which killed Nephrite and took his Black Crystal, though the Sailor Senshi destroyed the Youma. Zoisite took up the reigns immediately after Nephrite's death, where he used the Black Crystal to locate individuals with the Seven Rainbow Crystals inside of them. Unlike preceding commanders, Zoisite never bothered creating any civilian disguise. When he found his target, he would take the first opportunity to extract the Rainbow Crystal. He was able to successfully obtain some of them, however Tuxedo Mask shared this victory as well.
Eventually Zoisite challenged Tuxedo Mask to a duel for the Rainbow Crystals, under orders from Queen Beryl to bring him to the Dark Kingdom. However, Zoisite deliberately disobeyed Queen Beryl by attempting to immolate Mamoru Chiba in an elevator (ironically bringing Usagi with him by mistake), then stabbing him with an ice crystal, nearly killing him. When the Rainbow Crystals united and merged into the Silver Crystal, Sailor Moon transformed into Princess Serenity and defeated Zoisite with Moon Healing Escalation. Upon his return to the Dark Kingdom, Zoisite was killed by Queen Beryl for his disobedience. He died in Kunzite's arms as Queen Beryl informed Kunzite to let Zoisite's death be a warning to him if he ever harms Prince Endymion.
Near the end of the first season, Zoisite was seen in a flashback of when the Dark Kingdom invaded the Moon Kingdom, standing beside his fellow Shitennou members. He was also mentioned by a dying Kunzite, who knew he was about to meet him on the other side.
Zoisite teleported with an effect of petals surrounding him. He used these petals to also distract Tuxedo Mask and the Sailor Senshi, and he was also capable of transforming them into needles. He has displayed abilities of pyrokinesis as he tried to incinerate Usagi and Mamoru on an elevator. He could also conjure up shards of ice.
- Zoisite's name is likely derived from a mineral known as zoisite.
- Zoisite was the only Shitennou who both owned Youma and created other Youma from human beings.
- In the Russian dub, Zoisite was mistakenly called "Jadeite" in the early episodes.
- When attacking his foes or using the Black Crystal, Zoisite has a verbal tic of shouting the first syllable of his name.
- Zoisite's gender changed in several of the dubs, mainly due to his homosexual relationship with Kunzite being considered inappropriate for the target audience of the dubs. The Viz Media English, Portuguese, Spanish, French, Russian, Greek, Hungarian, Korean, and Cantonese dubs, as well as the Polish version, were among the ones in which Zoisite remained a man. In the Swedish dub, he was also female but in this case it was because the translators found his original Japanese voice so feminine they didn't realize he was a man in the first place.
- Zoisite makes a brief cameo in episode 70 of the anime; when Koan hits Yuuichiro, a chibi of him can be seen.
- He and Kunzite were the first same sex couple followed by Sailors Neptune and Uranus.
- His rivalry with Nephrite is similar to that between Rubeus and Esmerald later in Sailor Moon R. Zoisite often mocks Nephrite for his repeated failures much like how Esmerald mocks Rubeus for his failures.