Zoisite is the third of the Shitennou to appear. Zoisite is the commander of the Dark Kingdom's European division, as he states in Act 6. He is about the same height as Jadeite, and his estimated age is 16-17 according to the Materials Collection.
Zoisite has light long blond hair which he keeps in a pony tail. He wears a uniform, the same as Jadeite and Nephrite.
He first appears in Act 3 with Nephrite, pondering the appearance of the Sailor Senshi, although he is not named until Act 5, after Nephrite's death. After Nephrite falls, Kunzite warns Zoisite that he must be careful, lest the same thing happen to him, and the reputation of the Shitennou be tarnished.
The Dark Kingdom finds out that Sailor Moon is also seeking the Silver Crystal, and Queen Beryl instructs the Shitennou to obtain the Silver Crystal in order to provide energy to their great ruler, Queen Metaria. Zoisite vows to do the job. Zoisite disguises himself as a woman who is researching the crystal, Professor Izono, and appears on television claiming that the Silver Crystal has the power to provide eternal youth, and hypnotizes viewers into frantically searching for it. When the Silver Crystal is not found, Zoisite begins using television sets to steal energy from viewers. Usagi's energy is stolen, but Tuxedo Mask comes to her rescue. Sailor Mercury, Sailor Mars, and Sailor Jupiter confront Zoisite at the television station, but he vanishes before they can fight. Sailor Moon then uses her new recovery power (employing the Moon Stick) to save people.
In Act 7, Zoisite begins suspecting that Sailor Moon's power stems from the fact that she has the Silver Crystal, so he starts a video store called Rental Shop Dark. All of the videos in the store brainwash viewers with a subliminal message, inciting them to capture Sailor Moon and get the Silver Crystal. The students of Juuban Junior High school are acting strangely, so the senshi check the store out. They discover the hidden message on the tapes, and Sailor Moon heals those who watched the tapes with Moon Healing Escalation. The senshi finally reach Zoisite, but Sailor Moon's Moon Twilight Flash attack doesn't work on him. He grabs Sailor Moon from behind in chokehold, and demands that she tell him where the Silver Crystal is. Just as he is about to stab her, a boomerang knocks the knife out of his hand, and moments later, and second boomerang slices him apart. The boomerangs were those of Sailor V.
Later, when the Silver Crystal appeared, it was revealed that during the Silver Millennium, Zoisite and the other Shitennou originally were not evil - they served under Prince Endymion as his guardians and advisors. They were, however, tempted by the power of the Dark Kingdom (like most other humans), and fell to it. They eventually become good again, and their spirits advise Mamoru when he needs their help.
Zoisite also appears later in the short side story "Casablanca Memories." He again disguises himself as a woman - this time, he is a piano player at the Rain Tree restaurant. The Dark Kingdom creates music boxes which cause people to remember the past when they hear the music - the boxes drain the energy of humans. One is controlling Makoto, and Rei destroys it. She transforms into Sailor Mars, goes to the Rain Tree restaurant, and attacks Zoisite - but Zoisite disappears, angry that he could not avenge Jadeite's death.
Zoisite's name is likely derived from a mineral known as zoisite.
- According to Naoko Takeuchi, because Zoisite was so popular with fans of the anime she included him in the side-story "Casablanca Memory."
- He is the only member of the Shitennou who was not killed by his paired Sailor Senshi (Sailor Mercury); He instead was killed by Sailor Venus. while Jadeite was destroyed by Sailor Mars, and as for the other pairings, Nephrite was destroyed by Sailor Jupiter. both in their respectively original pair. Kunzite, on the other hand was killed from the Sailor Planet Attack, in which Sailor Venus was a part of.